https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53018 Wed 28 Feb 2024 16:09:22 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53356 Wed 28 Feb 2024 15:24:08 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38878 n = 13) erythrocyte-derived (CD235a) extracellular particles were increased, while platelet-derived (CD41b), leukocyte-derived (CD45), and CD4⁺T cell-derived (CD4) extracellular particles were decreased compared to both healthy controls (n = 27) (p<0.05) and secondary progressive multiple sclerosis patients (n = 9) (p < 0.05). Endothelium-derived extracellular particles (CD146) were increased in stable relapsing-remitting multiple sclerosis patients (n = 17) compared to healthy controls (p < 0.05). Extracellular particles from several different cells of origin correlated with each other and clinical parameters (e.g. disease duration, number of relapses, EDSS), though clinical correlations did not withstand corrections for multiple comparisons. Conclusions: Concentrations of erythrocyte-, leukocyte-, and platelet-derived extracellular particles were altered in relapsing multiple sclerosis patients and endothelium-derived extracellular particles were increased in stable relapsing-remitting patients compared to healthy controls. Extracellular particles may provide insights into altered the crosstalk between peripheral blood cells in multiple sclerosis, which may lead to the discovery of novel therapeutic targets.]]> Wed 23 Feb 2022 11:05:08 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39262 Wed 22 Jun 2022 19:13:42 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40650 Wed 13 Mar 2024 08:56:04 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28831 Wed 11 Apr 2018 13:23:36 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54539 Tue 27 Feb 2024 20:41:38 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54475 Tue 27 Feb 2024 14:56:31 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50644 Tue 01 Aug 2023 10:11:43 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51863 Thu 21 Sep 2023 10:16:17 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32442 Thu 09 Dec 2021 11:04:09 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38628 + T cells of relapsing–remitting (RR) MS patients compared to healthy controls and identified differentially methylated regions (DMRs) in HLA-DRB1 and RNF39. This study aimed to investigate the DNA methylation profiles of the CD4⁺ T cells of progressive MS patients. DNA methylation was measured in two separate case/control cohorts using the Illumina 450K/EPIC arrays and data was analysed with the Chip Analysis Methylation Pipeline (ChAMP). Single nucleotide polymorphisms (SNPs) were assessed using the Illumina Human OmniExpress24 arrays and analysed using PLINK. Expression was assessed using the Illumina HT12 array and analysed in R using a combination of Limma and Illuminaio. We identified three DMRs at HTR2A, SLC17A9 and HDAC4 that were consistent across both cohorts. The DMR at HTR2A is located within the bounds of a haplotype block; however, the DMR remained significant after accounting for SNPs in the region. No expression changes were detected in any DMRs. HTR2A is differentially methylated in progressive MS independent of genotype. This differential methylation is not evident in RRMS, making it a potential biomarker of progressive disease.]]> Mon 29 Jan 2024 17:52:47 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47575 Mon 23 Jan 2023 14:00:36 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45818 Mon 07 Nov 2022 12:18:03 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40033 Fri 15 Jul 2022 10:11:14 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:46091 Fri 11 Nov 2022 09:31:14 AEDT ]]>